| Summary: | 碩士 === 輔仁大學 === 食品營養學系 === 89 === The objectives of this study were to investigate the effects of pumpkin seeds and chromium yeast on the levels of plasma glucose and serum lipids in hyperglycemic rats induced by streptozotocin (STZ) and to evaluate that whether pumpkin seeds and chromium yeast supplements have synergistic effect. Fifty-six male Sprague Dawley (SD) rats were employed in this study. Forty-eight of them were intraperitoneally injected with STZ (20 mg/kg body weight, 2 times a week for 4 weeks) and fed high fructose diet to induce hyperglycemia. The hyperglycemic rats were randomly divided into six groups, 8 in each, and fed with different experimental diets, including hyperglycemic control (C), hyperglycemic positive control (PC, 500 mg Metformin/kg body weight/day), low pumpkin seeds (LPS, 20%), high pumpkin seeds (HPS, 40%), chromium yeast (Cr, 0.3%), high pumpkin seeds + chromium yeast (HPS+Cr). The other eight normal rats were fed rat chow. Animals were fed for 4 weeks. Our results showed that nonfasting plasma glucose levels were significantly decreased and impaired glucose tolerance in the hyperglycemic positive control, low pumpkin seeds, high pumpkin seeds, chromium yeast, high pumpkin seeds + chromium yeast than in the hyperglycemic control group (p<0.05). Nevertheless, hyperglycemic positive control and chromium yeast had increased plasma insulin levels after oral glucose ingestion (p<0.05). But fructosamine and total glycerated hemoglobin were not significantly affected. On serum lipid aspect, serum total cholesterol, triacylglycerol, LDL-cholesterol, HDL-cholesterol and free fatty acids had no significantly differences between hyperglycemic control and experimental groups (p>0.05). Besides, the antioxidant capacity to inhibit Cu2+-induced LDL oxidation and 50% hemolysis of red blood cells did not significantly delay oxidation times (p>0.05). But liver TBARS were significantly reduced in the hyperglycemic positive control and chromium yeast groups than in the hyperglycemic control group (p<0.05). In conclusion, pumpkin seeds and chromium yeast significantly decreased nonfasting plasma glucose and improved oral glucose tolerance test, but not influenced serum lipids and antioxidant capacity. It was also observed that chromium yeast had another benefits of increasing plasma insulin and reducing liver TBARS. Supplementation of pumpkin seeds and chromium yeast together didn’t show synergistic effect.
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