| Summary: | 碩士 === 逢甲大學 === 化學工程學系 === 89 === Abstract
Our recent work shows that Pseudomonas luteola can effectively decolorize an azo dye (Reactive Red 22). When the bacterial strain is used as the biocatalyst in practical decolorization process, the cell concentration in the system critically affects the efficiency and capacity of decolorization treatment. However, in batch or continuous cultivation, the cell concentration is usually limited to less than 2 g/L, resulting in a low decolorization efficiency. Therefore, this research applied fed-batch strategy to achieve high-cell-density cultivation of Pseudomonas luteola to enhance the decolorization performance. The experimental results show that medium composition and feeding strategy of medium had a close relationship with cell concentration. Through proper cultivation procedures, the cell concentration can be promoted to over 15g/L. Anaerobic decolorization operation was started after biomass concentration exceeded 15 g/L. The high cell density can significantly facilitate the capacity and applicability of the microbial decolorization bioreactor. Appropriate manipulation of dye concentration, feeding rate and treatment time was able to enhance the effectiveness of the decolorization bioprocess. In addition, repeated fed-batch decolorization was conducted to evaluate the stability of using the strain in decolorization operations.
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