Studies of Soybean Sprout Extract to Antioxidant

• Main Author: 李宜珊
• Other Authors: J.R. Too
• Format: Others
• Language: zh-TW
• Published: 2001
• Online Access: http://ndltd.ncl.edu.tw/handle/50841437760741619766

碩士 === 大葉大學 === 食品工程研究所 === 89 === Soybean sprout extract contain a lot of isoflavone, which are strong antioxidants. Isoflavone can scavenge reactive oxygen species to prevent cancer and cardiovascular diseases of human. In addition, isoflavone, just like estrogen, can help women to decrease the symptoms of menopause. The antioxidant ability and protective potential of lipid peroxidation (LPO) of soybean sprout extract were firstly determined in vitro. In the effect of scavenging ,-diphenyl--picryhydrazyl (DPPH) free radical, experimental results showed that soybean sprout extract are as good as Vit E and Vit C. In the test of reducing ability of redox, results showed that soybean sprout extract have a strong ability to reduce Fe3+ of potassium ferricyanide. By using the thiobarbituric acid reactive substances (TBARs) test, soybean sprout extract have been shown to possess an ability to prevent low-density lipoprotein from being oxidized. After a series of in vitro tests, soybean sprout extract were evaluated to possess antioxidant ability. Therefore, soybean sprout extract were used for further studies on animals. There was no evidence to show that the addition of SBE could rescure the effect of decreasing activity of swine sperms due to the existence of dithyl-dithiocarbamic acid (DDC), which is a superoxide dismutase (SOD) inhibitor. DDC was also added into the feed of mice to evaluate their effects in biofunctions. The glutathione peroxidase (GSH-Px) and catalase (CAT) contents in the livers of mice supplemented with DDC were significantly less than those in the livers of mice without supplemented with DDC. Furthermore, the intake of DDC also cause mice to have less embryos and pup number per farrow (P < 0.05). If both SBE and DDC coexist in the feed, the above situations could be significantly improved. For instance, a feed with 2% of SBE can reduce the concentration of blood lipid in rat. The concentrations of plasma total cholesterol and triglyceride of the rats taking a feed with 2% of SBE were less than those in the control group by 22% (P < 0.05) and 42% (P < 0.01), respectively. Based on the experimental results performed above, we concluded that SBE is an efficient antioxidant and can elevate antioxidant status and increase reproduction performance of mouse. SBE supplement can reduce the concentration of blood lipid of rat. Further studies are needed to prove that SBE may have some other biofunctions.