| Summary: | 碩士 === 中國醫藥學院 === 醫學研究所 === 89 === To evaluate the relationship between protein kinase C (PKC) and vascular endothelial growth factor (VEGF) gene expression in hepatocellular carcinoma, 15 patients who had undergone curative hepatic resection for HCC in the past one year were enrolled into this study, VEGF and PKC gene expression were evaluated for HCC and the liver by semiquantitave reverse-transcription polymerase chain reaction (RT-PCK) and immunostaining. The results of these assays were compared with the patient’s general data, clinical manifestations and laboratory data and pathological features.
Tumoral stain on angiography was significantly correlated with VEGF expression in HCC by immunostaing (P=0.0003), however, tumor size, histological grade of malignancy, the level of α-feto protein, portal vein invasion, and the microscopic infiltration of cancer cells into the tumor capsule were not correlated with VEGF expresion in HCC. VEGF was detected in tumor and surrounding liver tissues. VEGF and PKCβ1 were also found in tumoral microvessels by immunostaing. The OD values of VEGF mRNA and PKCβ1 mKNA, mHCC were not correlated by RT-PCR assay. The OD values of VEGF mRNA and PKCβ1 mKNA were not correlated be tween tumor and non-tumor portions.
In conclusion, VEGF play an important role in Angiogenesis of HCC, PKCβ1 lies on the pathway by which VEGF mediated this reaction. A larger series of studies are necessary to elucidate its clinic implication in HCC.
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