Determination of urinary 8-Hydroxy-2''-deoxyguanosine in breast cancer patients

• Main Authors: Hu John Wen, 胡宗文
• Other Authors: KUO HSIEN-WEN
• Format: Others
• Language: zh-TW
• Published: 2001
• Online Access: http://ndltd.ncl.edu.tw/handle/08489368166461952561

碩士 === 中國醫藥學院 === 環境醫學研究所 === 89 === Abstract Oxygen radicals have been shown to cause extensive damage to DNA, which it leads to gene mutations. Urinary 8-hydroxy- 2’-deoxyguanosine（8-OHdG）has been widely studied as a biomarker for detection oxidative stress as it is one of the most specific forms of DNA damaged by reactive oxygen species. 8-OHdG is thought to be promutagenic lesion since this lesion induces G：C to T：A transversion unless repaired prior to replication. The objective of this study is to compare the urinary 8-OHdG levels between breast cancer patients and healthy women. Our results indicated coffee drinking did not significantly correlate with 8-OHdG in healthy women, but it correlated with urinary 8-OHdG levels in breast cancer patients. There were significant higher urinary 8-OHdG levels in breast cancer patients（81.81nM）compared to healthy women (33.14nM). Urinary 8-OHdG levels after creatinine and body weight corrected were compared between breast cancer patients and control women the results were the similar. When the point of urinary 8-OHdG levels was cut at 45nM based the receiver operational characteristics, the sensitivity and specificity for diagnosis of breast cancer were 69﹪and 75%, respectively. When the point of 8-OHdG levels was cut at 36nM for classification between the benign and malignant breast tumor, the sensitivity and specificity for diagnosis of breast cancer were 73﹪and 80%, respectively. Urinary 8-OHdG levels in stage I of cancer was higher than that of stageⅡ and stageⅢ of cancer, however, it was not significantly. Our concluded urinary 8-OHdG is used to as a biomarker of oxidative stress in breast cancer.