| Summary: | 碩士 === 國立陽明大學 === 醫學工程研究所 === 88 === The treatment of bone fracture aims at early and complete restoration of limb function. Internal fixation achieves stable fixation using metal implants which help to maintain the anatomical reduction and carry more of the load than the bone. Thus, stress protection is shown by an increase in the medullary diameter. Since bone is weakened by this remodeling, internal fixation, such as plates & screws should be removed within 18-24 months. The disadvantage of such treatment illustrates the need of new materials which degrade within the body to harmless metabolites and maintain the fixation strength during bone healing process.
Our research is focus on the tissue response to PLGA (poly lactic glycolic acid) copolymer manufactured by Industrial Technology Research Institute in mice model. The research design is followed te principles of Biocompatibility Tests in Iso 10993. Forty BALB/c mice are randomly divided in 4 groups of 5 animals each according to the experimental time (1, 3 months). PLGA rods, k-wires and catguts are inserted between the hamstring muscles and femoral bone. Sham operation is performed as negative control. The fibrous capsules around implants and spleens are harvested and examined under microscopy. Besides, cytokines (IL-6, TNF-α) are analyzed via RT-PCR technique. The tissue response is no obvious difference between PLGA rod and k-wire group in histomorphological picture. While the positive rate of IL-6 expression, the intensity of PLGA group is the least one than the k-wire and catgut group. The preliminary results reveal that PLGA is quite immunologic inert and good tissue tolerance in mice model.
|
|---|
