Molecular cloning and analysis of two promoters and two cDNA encoding CTR1 like protein kinase from broccoli floret

• Main Authors: Tzu-I Chaw, 趙子儀
• Other Authors: Jei-Fu Shaw
• Format: Others
• Language: zh-TW
• Published: 2000
• Online Access: http://ndltd.ncl.edu.tw/handle/34451541226602695712

碩士 === 國立陽明大學 === 生物化學研究所 === 88 === Two Broccoli CTR1 (Bo-CTR1) genes cloned by using homology-based PCR technique encode two open reading frame of 808 and 799 amino acids. The mRNA transcripts of two CTR1 genes were analyzed by RT-PCR technique. In ethylene treated broccoli floret, Bo-CTR1 A gene expression was induced by ethylene and the expression level was higher than that of BO-CTR1 B. In the process of broccoli post-harvest senescence, the expression of Bo-CTR1 B reached to a constant level in leave after 1 day. The expression level of Bo-CTR1 A was gradually increased over 5-days of post-harvest treatment. No difference was identified between the expression of Bo-CTR1 A and Bo-CTR1 B during this peroid. In order to realize differential expression of these two Bo-CTR1 genes, the promoter region of Bo-CTR1 A and Bo-CTR1 B were cloned with the size of 2.8 kb and 0.8 kb, respectively. Further characterization on the promoters and gene regulation mechanisms of Bo-CTR1 A and Bo-CTR1 B should be carried out. 中文摘要 ……………………………………………………………… ii 英文摘要 ……………………………………………………………… iii 縮寫表 …………………………………………………………………… iv 目錄 ……………………………………………………………………… v 表目錄 …………………………………………………………………… vi 圖目錄 …………………………………………………………………… vii 緒論 ……………………………………………………………………… 1 材料與方法 ……………………………………………………………… 5 結果 …………………………………………………………………… 16 討論 …………………………………………………………………… 20 回顧與展望 …………………………………………………………… 23 參考文獻 ……………………………………………………………… 24 表 ……………………………………………………………………… 26 圖 ……………………………………………………………………… 27