Cataract Preventive Agents from Nature Products

• Main Authors: wang Yi -Jen, 王怡珺
• Other Authors: Prof. Ling-Ling Yang
• Format: Others
• Language: zh-TW
• Published: 2000
• Online Access: http://ndltd.ncl.edu.tw/handle/17986486378329786744

碩士 === 台北醫學院 === 生藥學研究所 === 88 === Cataract is mainly response of decreased visual acuity and the blindness among this population. The accumulation of sorbitol and the lipid peroxidation in the lens is the major cause of cataract. The anticataract effects of nature products are explored in this studies. There are five thousands of plant species in Taiwan, in the first portion of this study, twenty-eight plants with abundant polyphenols were used to study the inhibition effects on the rat and pig aldose reductase. The inhibition of the rat aldose reductase of Eugenia uniflora Linn., Camellia candata Wall. , Camellia nokoensis Hay., Camellia tenuifolia (Hay.) Cohen-Stuart., Coptis chinensis Franch were 100 %, 82.55 %, 87.60%, 61.63 %, 100 % in the 20 g/ml conc. And the inhibition of the pig aldose reductase of Eugenia uniflora Linn., Camellia candata Wall. , Camellia nokoensis Hay., Camellia tenuifolia (Hay.) Cohen-Stuart., Coptis chinensis Franch were 100 %, 67.17 %, 87.77 %, 75.34 %, 56.20 % in the 20 g/ml conc. Furthermore, twenty-two kinds of nature flavonoids were used to study the inhibition effects on pig aldose reductase. The IC50 compare with quercetin ( positive control ；IC50 =5.05×10-2mM) of Quercitrin, Quercetin pentaacetate, Baicalein, Neohesperidin, Edultin, Myricetin, Baicalin, Nobiletin, and Morin were1.1×10-2 mM, 2.08×10-2mM, 2.21×10-2 mM, 2.47×10-2 mM, 3.30×10-2 mM, 3.48×10-2 mM, 3.60××10-2 mM, 3.97×10-2 mM, and 4.27×10-2 mM. In the next portion of this study, the same twenty-two kinds of nature flavonoids were used to study the inhibition effects on lens lipid peroxidation. The IC50 compare with Vit. E（positive control ；IC50=9.15×10-1 mM）of Quercetin pentaacetate, Quercitrin, ﹑Neohesperidin, Rutin, Fisetin, Kaeompferol, Naringin, Nobiletin, Edultin, Luteolin, Tangeretin, Apigenin, Fraxi, Wogonin﹑and Oroxylin A were 1.85×10-1 mM, 2.10×10-1 mM, 3.04×10-1 mM, 3.12×10-1 mM, 3.20×10-1 mM, 3.21×10-1mM, 3.23×10-1 mM, 3.31×10-1 mM, 3.63×10-1 mM, 3.66×10-1 mM, 4.27×10-1 mM, 4.28×10-1mM, 4.72×10-1 mM, 4.91×10-1 mM, and 5.84×10-1 mM when ues the NADPH to be the inducer. And the IC50 compare with Vit. E（IC50 =1.52×10-1 mM）of Neohesperidin, Rutin, Nobiletin, Quercitrin, Naringin, Esculin, Baicalin, Quercetin pentaacetate, and Edultin were 0.90×10-1 mM, 1.04×10-1 mM, 1.10×10-1 mM, 1.10×10-1 mM, 1.23×10-1 mM, 1.32×10-1 mM, 1.30×10-1 mM, 1.40×10-1 mM, 1.47×10-1 mM when the Fe2+ was the inducer. Key words: Cataract, Aldose reductase, Lipid peroxidation, Natural product, flavonoids