| Summary: | 碩士 === 台北醫學院 === 生藥學研究所 === 88 === Injuries arising from sports, the workplace, and traffic mishaps, and others of an accidental nature, are on the increase in today’s busy commercial society. In fact, according to the reports of Legislative Yuan’s Ministry of Health, accidental injury is already ranked as the fourth highest cause of death nationwide. Through medication, surgery, and physical therapy, many patients can achieve a full recovery. However, those suffering from nerve axon damage require an extended period of convalescence and full recovery is commonly unattainable. As such, the research into drugs promoting nerve regeneration and repair has become a major research goal within modern medicine. This project selected a frequently employed Chinese Bu Qi (補氣) medical prescription —Bu Zhong Yi Qi Tang (補中益氣湯, BT)─and conducted a systematic appraisal of it.
The development and assessment of medicines stresses efficacy and safety foremost; but even more important is consistency BT was then freeze-dried to create a concentrate convenient for use in the laboratory. Next, the Thin Layer Chromatograph ( TLC ) analytical method was applied to ensure consistent quality of the ingredients.
In this experiment, rats were applied with WGA-FITC (Lectin from Triticum Vulgaris) to assess the effects of BT upon nerve regeneration. Rat sciatic nerve were damaged with liquid nitrogen. Then the rats were treated with BT for 45 days. After the recovery period, the rats were injected WGA-FITC, which was uptaken by the regenerating nerve axon and retrograde to the cell body of neurons. We employed microscopes to observe the number of FITC-labeled neurons.
The results showed that the number of labeled regenerating neurons of the experimental group were 4.1, 4.5, 4.4 and 5.1 per section of the lumbar spinal cord, and the control group were 3.2, 3.6, 3.4 and 3.8 (P< 0.05) , These results demonstrated that BT may be useful in promoting nerve regeneration and deserve for further study in the future.
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