Antiproliferation effects of Gelidium amansii extracts on cultured cells

• Main Authors: Ching-Jung Tu, 杜青蓉
• Other Authors: Yue-Hwa Chen
• Format: Others
• Language: zh-TW
• Published: 2000
• Online Access: http://ndltd.ncl.edu.tw/handle/47544325394409831068

碩士 === 台北醫學院 === 保健營養學研究所 === 88 === The objective of this study was to investigate the effect of Gelidium amansii on the cell growth and the potential role of apoptosis on the growth inhibition was also explored. Two lines of cancer cells, murine hepatoma cell (Hepa1) and human promeyolitic leukemia cell (HL-60), and one normal cell lines, murine embryo fibroblast cell (NIH3T3) were used in this study. The cells were treated with PBS-, methanol-, or DMSO extracts of Gelidium amansii, and the cell growth and the cell proliferation were measured. The results indicate the PBS - soluble extract did not have the effects on the all lines of cells, whereas methanol-soluble extract had the inhibitory effect on the Hepa1 and NIH3T3 cells (p＜0.05), and DMSO- soluble extract had inhibitory effects on all lines cells (p＜0.05). The methanol-soluble extract treated Hepa1 and NIH3T3 cells, and all lines of cells treated with DMSO-soluble fraction of the Gelidium amansii showed annexin V positive response, and had fragmented DNA ladders on agarose gels indicating that the cells underwent apoptosis. Gelidium amansii possesse the antiproliferation effect of cultured cells and this may due to the induction of the apoptosis pathway, this inhibitory effect did not show cell specificity.