Effects of Chlorella on the Antioxidative Status in Rat

• Main Authors: Chia-Yi Yuan, 袁家儀
• Other Authors: Jiun-Rong Chen
• Format: Others
• Language: en_US
• Published: 2000
• Online Access: http://ndltd.ncl.edu.tw/handle/53723559871961204501

碩士 === 台北醫學院 === 保健營養學研究所 === 88 === This study was to evaluate the antioxidant effect of chlorella on the concentrations of the antioxidants, the activities of the antioxidative enzymes, the antioxidative capacity, and the total antioxidant status in Sprague-Dawley rats after six-week oral feeding. The first experimental results showed that 0, 1,5, or 10% chlorella diet did not affect normal growth of the rats. Different dosages of chlorella diets didn’t affect levels of ferrous ion, ferric ion, and the ratio of ferrous to ferric ions, but significantly elevated the activity of glutathione peroxidase in the erythrocytes and liver. The activities of erythrocyte glutathione reductase and superoxide dismutase were significantly elevated by 1% and 5% chlorella diets. Addtionally, the concentrations of lipid peroxidation products in the low density lipoproteins were reduced in the rats fed 10% chlorella diet, but were elevated by 10% chlorella diet in the liver. The second experimental results showed that the levels of plasma ferrous ion were reduced by 50~70% in the rats fed 0.5 or 4% chlorella diet. All of the chlorella diets (0.1, 0.5, and 4%) significantly elevated the levels of plasma ferric ion and total iron, and decreased the ratio of ferrous to ferric ions in the plasma. The activities of erythrocyte glutathione reductase were increased and those of hepatocyte superoxide dismutase were decreased by all of the chlorella diets. The activities of erythrocyte superoxide dismutase were significantly elevated to 1.5-fold by 0.5 and 4% chlorella diets. However, different dosages of chlorella diets did not affect the activities of erythrocyte glutathione peroxidase and catalase, and those of hepatocyte glutathione reductase and catalase as well. The concentrations of lipid peroxidation products in the low density lipoproteins were reduced by 13, 68, and 57% in the rats fed 0.1, 0.5, or 4% chlorella diet, respectively. However, the concentrations of lipid peroxidation products in the liver were not affected by the chlorella diet. The total antioxidant status in the plasma was one-fold greater in the rats fed the chlorella diet than the control group. In summary, the chlorella diet increases antioxidative capacity in the rats, especially 1 and 5% chlorella diets improve antioxidative capacity more efficiently than 10% chlorella diet, and 0.5 and 4% chlorella diets improve antioxidative capacity more efficiently than 0.1% chlorella diet. The third experimental results showed that 0.5% chlorella diets can not improve antioxidative capacity in the rats treated with CCl4 under oxidative stress.