| Summary: | 碩士 === 台北醫學院 === 生物醫學技術研究所 === 88 === Alzheimers disease (AD) is one of the most important cause of the Dementia. In Taiwan, with the people populations aging, ADs patients are also continue increasing. Many factors may be involved in the pathogenesis of AD, for example, family history, sex and estrogen, etc. Up to now, a variety of studies have shown that genetic factor plays an important role in AD. In this study, we collected a total of 192 samples including 82 sporadic late- onset AD patients, and 110 health elders controls as samples. Different statistic programs were used to analyze the possible interaction of 9 AD related genes. The 9 sets of AD related genes include apolipoprotein E 4 (ApoE-4), promoter of apolipoprotein E (ApoE, G-186T), regulatory region of apolipoprotein E (ApoE, A-491T), mutation of α2-macroglobulin (A2M G2998A), five nucleotide deletion of α2-macroglobulin, bleomycin hydrolase (BH, A1450G), presenilin-1 intronic polymorphism (PS-1, allele 1/2 ), 1- antichymotrypsin (ACT, A/T), low-density receptor-related protein (LRP, C766T). PCR-RFLP (Polymerase chain reaction-restriction fragment length polymorphism) was used to analyze the polymorphisms or mutations of these 9 sets of AD related genes.
The results showed that only ApoE 4 significantly increases the risk of AD using these 9 sets of related genes. However, besides ApoE4, other 8 sets of related genes polymorphisms or mutations are not the factors to cause AD in Taiwan.
Taken together, AD patients in Taiwan may have other different high risk factors. Continue searching for other polymorphisms or mutations of AD related genes or other candidates may be helpful in understanding the mechanism of AD pathogenesis.
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