Identification of SMN -interaction proteins by yeast two-hybrid system

• Main Authors: Yun-Ting Chiu, 邱韻庭
• Other Authors: Hung Li
• Format: Others
• Language: zh-TW
• Published: 2000
• Online Access: http://ndltd.ncl.edu.tw/handle/70602222623158029428

碩士 === 中國文化大學 === 生物科技研究所 === 88 === Spinal muscular atrophy (SMA) is a common autosomal recessive disorder that results in the degeneration of spinal motor neurons. SMA is categorized as types I-III, ranging from severe to mild, based upon age of onset and clinical course. Survival motor neuron (SMN) gene is the SMA-determining gene that is a ubiquitously expressed gene, which has been implicated in RNA processing. However, how the function of SMN relates to the specific motor neuron death remains unsolved. In an attempt to identify SMN-protein interactions relevant to the specific nature of the motor neuron degeneration characteristic of SMA, we carried out yeast two-hybrid system using the entire coding sequence of the human SMN gene as bait in screening a human fetal brain cDNA library. Four cDNAs interacting with SMN were isolated for several times: BARD1, FBP, PML, TALLA1. These associated proteins will be confirmed using in vitro binding and in vivo co-immunoprecipitation assays. The relevance of these proteins to the pathology of SMA will be further explored.