Safety Evaluation of Recyling Piggery Treatment Eggluents after Ultraviolet Sterilization and Chlorine Disinfection Processings

• Main Author: 余崇業
• Other Authors: 周晉澄
• Format: Others
• Language: zh-TW
• Published: 2000
• Online Access: http://ndltd.ncl.edu.tw/handle/61582981965395901951

碩士 === 國立臺灣大學 === 獸醫學研究所 === 88 === 　　The aims of this study were first to evaluate the bactericidal efficiency of both ultraviolet (UV) radiation and chlorination through biological pollution indicators, fecal coliforms (FC) and fecal streptococci (FS), in order to assess the safety use of piggery treatment effluents and to realize the bactericidal intervention impact on the FS distribution and its antibiotic resistance profile. 　　Piggery effluents from Chunan A wastewater treatment facility (Chunan A) were treated by UV radiation at different time period with UV intensity of about 1 mW/cm2 at 254 nm. For chlorination evaluation, piggery effluents from Chunan B wastewater treatment treatment facility (Chunan B) were treated by sodium hypochloried with free residual chlorine (FRC) concentration of 0 mg/L to 25 mg/L and with chlorination time of 0 to 15 minutes. 　　Results showed that most effluents of COD and SS from both plants could be complied with water quality standard of 1999. CODs from Chunan B effluents after Oct. 1999 were higher than the regulated standard were associated with the decteasing microbial activity from environmental temperature effects or by unstable electric power supply. UV radiation was a reliable sterilization tool for FC and FS in piggery effluents of underlined compliable effluents, and FCs were more fragiable than FSs. For 99.9% bactericidal efficiency, 84 mWs/cm2 UV254 dosage was effective for FS and 48 mWS/cm2 UV254 dosage was good for FC. Thus, FS is a better promisiable biological indicator for evaluation of the UV safety than FC. Chlorination is a good disinfection tool for FC control. While FRC up to 10 mg/L and 12 minutes disinfection time, the FC was effectively killed. However, the lowest surival fraction of FS was only-1.32 when the maximum chlorination dosage, FRC 25 mg/L--15 minutes, was applied. FS can serve as a good indicator for the evaluation of the chlorination of piggery effluent, while FC can be used to monitoring the chlorination efficiency purpose. 　　For bactericidal intervention evaluation of FS distribution, effluents were treated by UV for 300 seconds of by 10 mg/L FRC for 12 minutes. The isolated FS strains were further identified by API rapid id32 strep identification system and then performing the selected 18 antibiotic sensitivity tests. 　　High dose UV sterilization could alter FS''s composition and increase the resistance to the antibiotics. Chlorination caused similar FS strain pattern between Chunan A and Chunan B. However, FS isolated from Chunan A after chlorination were more resistant to ceftriaxone, cephalothin, kanamycin and peniclllin G, FS from the Chunan B were more sensityve to chlorampnenicol, gentamicin and kanamycin. Although the antibiotic resistance profile of both piggery wastewater share different characteristics after UV sterilization and also chlorination, the possibility of increasing antibiotic resistance after both meatment should be taken into consideration for the prevention of further public □ damage.