The Expression Distribution of tski mRNA in tilapia (Oreochromis aurea) During the Developmental Stages

• Main Authors: Li-Fen Lee, 李莉芬
• Other Authors: Huai-Jen Tsai
• Format: Others
• Language: zh-TW
• Published: 2000
• Online Access: http://ndltd.ncl.edu.tw/handle/00345990318763769182

碩士 === 國立臺灣大學 === 漁業科學研究所 === 88 === Overexpression of v-ski, or its proton-oncogene, c-ski, has an effect on proliferation, morphological transformation and myogenic differentiation in fibroblast culture and transgenic animal. As a transcription factor, the biological role of c-Ski depends on the cellular environment and is under deciphering. Two types of c-ski, tski1 and tski2, have been identified in tilapia, Oreochromis aurea. tski transcripts are widely expressed in different adult tissues and developmental stage embryos. The most and the least abundant tski transcripts are located in the brain and skeleton muscle, respectively. In situ hybridization analyzes the different type of adult tilapia muscles, revealing little expression of tski transcripts in the smooth muscle of intestine and stomach, heart muscle and skeleton muscle. However, tski2 transcripts are more abundant than tski1 transcripts in heart and skeleton muscle fiber. Meanwhile, tski transcripts are expressed in the mucosa of intestine and stomach. The gill filament and cortex of cerebrum express abundant tski. The cortex of brain expresses abundant tski transcripts in the stage 17 embryo (mouth opening and gill formation). The myotube, embryo muscle fiber (superficial red muscle and inner white muscle), cartilage in the gill arch and in the developing endochondral bone, and neuroretina also have the expression of tski transcripts. Meanwhile tski1 transcripts are more abundant in muscle fibers than tski2 transcripts. The fry (active swimming and feeding) tissues express high degree of tski transcripts in the cortex of brain, neuroretina. The intestine and stomach also express high tski transcripts, when the digestive system of fry is well developed.