Studies on the transferrin-like protein and gene of the green alga Dunaliella tertiolecta
博士 === 國立臺灣大學 === 海洋研究所 === 88 === The role of iron in the growth and physiology of the marine alga Dunaliella tertiolecta was investigated. Comparison of the electrophoretic profiles of total proteins from cells grown under iron-replete or iron-deficient conditions revealed that the abundance of a...
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| Format: | Others |
| Language: | zh-TW |
| Published: |
2000
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| Online Access: | http://ndltd.ncl.edu.tw/handle/63045349201840486614 |
| Summary: | 博士 === 國立臺灣大學 === 海洋研究所 === 88 === The role of iron in the growth and physiology of the marine alga Dunaliella tertiolecta was investigated. Comparison of the electrophoretic profiles of total proteins from cells grown under iron-replete or iron-deficient conditions revealed that the abundance of a 38-kDa protein was markedly increased in cells deprived of iron. Electron microscopy also revealed that cells subjected to iron limitation exhibited substantial chlorosis and a defective photosynthetic apparatus. In addition, immunoblot analysis showed that iron deprivation increased the abundance of a 150-kDa protein that reacts with antibodies to human transferrin. With the use of the polymerase chain reaction and primers based on the nucleotide sequence of a gene for a transferrin-like protein in D. salina, a 9.5-kb fragment of D. tertiolecta genomic DNA that encodes a homologous protein was isolated and sequenced. The Dunaliella tertiolecta gene comprises 30 exons and contains an open reading frame of 3825 bp that encodes a protein of 1274 amino acids. The predicted amino acid sequence of the encoded protein shows a high degree of homology to those of various transferrin proteins from other organisms.
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