| Summary: | 碩士 === 國立臺灣師範大學 === 化學研究所 === 88 === High-performance liquid chromatography (HPLC) and capillary electrophoresis (CE) are analytical methods most commonly used presently to quantify the components of Chinese herbal medicines.
The first section of this research, a HPLC method for Macroto- mia euchroma was established. The major components of M. euchroma are naphthaquinone derivatives, containing shikonin (SK)1, acetyl- shikonin(ASK) 2, teracrylshikonin(TSK) 3 , isovalerylshikonin (IVSK) 4 , isobutylshikonin(IBSK) 5, β,β-dimethylacrylshikonin (BSK) 6 . The RP-LC method was carried out within 55 minutes by using cosmosil C18-MS column and a gradient solvent system of acetic acid buffer-methanol-acetonitrile, and detecting at 280 nm. The effect of column selectivity, acetic acid concentraction and organic modifier concentraction of the mobile phase on the seperation of the analytes was also studied.
In this research, Macrotomia euchroma and Lithospermum erythror- hizon was collected for analysis, it was found that the absorptions of the six cpmponents in M. euchroma were clear, but the absorption of 3 wasn''t found. In addition, there were two clear absorptions in L. erythrorhizon : one was hydroxyisovalerylshikonin (marked 8 ) and the other was unknown (marked 7 ). The total amount of naphthaquinone derivatives in M. euchroma (80.28±39.75 mg/g) was higher than L. erythrorhizon(23.54±44.51 mg/g). The area ratio of 3/2 in M. euchroma was higher than 0.03, but about zero in L.erythrorhizon . In addtion, the area ratio of 7/2 was less than 0.1 for the former, and was higher than 0.2 for the latter; the ratio of 5/2 was higher than 0.4 for the former and less than 0.1 for the latter. From these datas on the chemical analysis of the herb''s constituents, we can postulate the origin and quality of the herb drug.
The second section of this research, a CE method for M. euchroma was established. We used "sweeping" as the analysis method, carried out within 40 minetes using a buffer system containing 110 mM SDS in 70 mM phosphoric acid and 50% methanol (pH = 1.85, cond.= 2.40), and detecting at 210 nm. Using this method, four components of M. euchroma were analyzed wherein ASK(2) and TSK (3) were over- lapped.
Comparing the HPLC and CE methods for M. euchroma, we found that the detection limit in sweeping was lower than in HPLC, except the theoretical plat number and the reproducibility which were better in HPLC than in sweeping.
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