Inhibition of Telomerase Activity by hTR Gene in Human J5 Hepatoma Cell Line

• Main Authors: Shou-Bin Chao, 趙壽彬
• Other Authors: Chung-Lung Cho
• Format: Others
• Language: zh-TW
• Published: 2000
• Online Access: http://ndltd.ncl.edu.tw/handle/49140176798903944867

碩士 === 國立中山大學 === 生物科學系研究所 === 88 === Telomerase, a ribonucleoprotein enzyme has its own internal RNA template that elongates telomeres and stabilizes chromosome structure. The mayority of hepatoma express high levels of human telomerase template RNA ( hTR ) that is essential for cellular telomerase activity. In this study, we examined whether hTR、antisence hTR and IFNα gene had a telomerase activity inhibitory effect on J5 hepatoma cell line, through transfection via an expression vector . The expression of mRNAs for hTERT、hTR、c-myc、IFNα、p16 and p27 were also detected. The results suggested that inhibition of telomerase activity and overexpression of hTR gene were observed in FhTR-treated J5 cells. The expression of mRNAs for hTERT、c-myc、IFNα、p16 and p27 genes were not changed whether telomerase was inhibited or not. In addition, genomic DNA fragmentation was observed in telomerase inhibited J5 cells indicating that apoptosis was undergoing in these cells.