The study of the isolated microbial divisity in the EBPR reactor

• Main Authors: Cheng Ming-Kui, 鄭閔魁
• Other Authors: Liu Wen-Tso
• Format: Others
• Language: en_US
• Published: 2000
• Online Access: http://ndltd.ncl.edu.tw/handle/16604905681658989348

碩士 === 國立中央大學 === 生命科學研究所 === 88 === Abstract: The basic principle of Enhanced biological phosphorous removal (EBPR) process is the anaerobic release and aerobic surplus uptake of phosphorus from wastewater by phosphorous accumulating organisms (PAOs). The existence of a stable PAO community is very important to maintain efficient treatment of phosphate. In this study, the traditional isolation method was used to isolate pure cultures from an anoxic-oxic process. 96 strains were isolated from an efficient phosphorous removal A-O process. These 96 strains were screened using DGGE of 16S rDNA and grouped into 34 groups according to the closeness of bands expressed on DGGE. After 16S rDNA sequencing, 21 distinctively different strains were identified. 32 bacterial strains belonged to theβ-subdivision Proteobacteria, 17 strains to theα-subdivision Proteobacteria. Moreover, members of Gram positive HGC, and Cytophaga- Flexibacter- Bacteroids. By further comparison with Gram positive LGC phylums were also isolated. 16S rDNA clone library study (Nielsen et al., 2000), only bacterial strains affiliated with Flavobacterium ferrugineum of the CFB group was common group found. This finding suggests that a polyphasic approach is useful in the determination of microbial community.