Detection of Chlamydophila abortus by polymerase chain reaction

• Main Author: 張澔任
• Other Authors: 馮翰鵬
• Format: Others
• Language: zh-TW
• Published: 2000
• Online Access: http://ndltd.ncl.edu.tw/handle/59334353234665564833

碩士 === 國立中興大學 === 獸醫微生物學研究所 === 88 === The purpose of this study was to develop a specific polymerase chain reaction for rapid detection of Chlamydophila abortus. We selected the published chlamydial omp A and 16S-23S rRNA gene sequences from GeneBank, and respectively aligned these sequences to find suitable regions for Chlamydophila abortus-specific primer design. The alignment of 16S-23S rRNA gene sequences revealed no suitable region for primer design, while the alignment of omp A gene　sequences revealed many suitable regions for Chlamydophila abortus- specific primer design. We designed several primer pairs from these suitable regions of omp A gene by Primer_3, and finally we selected 358f-868r, 433f-834r, 835f-1064r and 402f-1064r four primer pairs for the examination of detection of Chlamydophila abortus by polymerase chain reaction. The result showed that all the four primer pairs were specific for Chlamydophila abortus, so that our polymerase chain reaction with these primer pairs could permit the rapid and specific detedtion of Chlamydophila abortus, and the 358f-868r primer pair was the most sensitive pair. By this study we have developed a specific polymerase chain reaction for rapid detection of Chlamydophila abortus.