Applicability of Non-conventional Protein Feedstuff

• Main Authors: Ssu-Yu Chang, 張嗣瑜
• Other Authors: Prof. B. Yu, Ph D.
• Format: Others
• Language: zh-TW
• Published: 2000
• Online Access: http://ndltd.ncl.edu.tw/handle/53895152395728080337

碩士 === 國立中興大學 === 畜產學系 === 88 === Applicability of Non-conventional Protein Feedstuff in Broiler Diets The purpose of this study was to evaluate the changes in a non-conventional protein feedstuff with exogenous protease supplement using an in-vitro digestive trial contrasted to an in-vivo broiler trial. Soybean meal (SBM), sunflower meal (SFM) and canola meal (CM) were used as the protein feed source, with or without the same activity of bromelain (750 units / solids) or microbial protease (45 units / solids) administered in 9 treatments for a 3 × 3 factorial experiment. The results showed that protein and amino acid digestibility and the percentage of water-soluble protein of SFM after in vitro digestion was significantly higher than the other trial diets. There was a significant affect on amino acid digestibility form the exogenous proteases supplement. The water soluble protein distribution showed that the percentage of molecular weight greater than 150 kDa in the SBM was higher than the other trial proteins. The molecular weight less than 1 kDa in SFM was significantly lower than the other. The SDS - PAGE polypeptide distribution showed the same soluble protein trend. Ninety, fourteen day old and 45, thirty-five day old Arber Acer broilers used for the in vivo availability trial were randomly divided into 9 groups for a 3 × 3 factorial experiment. The experimental diet was formulated into an isonitrogenic semipurified diet with the same protein and exogenous proteases supplement used in the in—vitro trial. The result showed that the SFM protein and amino acid availability was higher than SBM and CM in the growth and finishing periods. The plasma total free amino acid concentration taken in the first hour after a meal had same trend as the availability data (P < 0.05). Compared with the control, the addition of microbial protease had a significant effect on improving protein and amino acid availability and total free amino acids in the plasma. The bromelain supplement showed no improvement on protein and amino acid availability. Seven hundred and four Arber Acer chicks used for the growth trial were randomly divided into 32 pens for a 4 × 2 factorial experiment. The treatment involved four levels of SFM protein substitute of SBM protein (0, 5, 10 and 20 %) in isoenergenic and isonitrogenic diets, with or without microbial protease supplement. Results showed that there were no significant differences in feed intake, efficiency and weight gain during the growing period between all treatments. During the finishing period and for the entire trial period, feed intake and weight gain increased with increasing levels of SFM in the diet. Addition of microbial protease to the diet had a significant effect on improving growth performance. In-vitro digestive trial and in-vivo availability trial showed SFM protein is hydrolysed by digestive enzymes more easily than the other protein source. We must think upon all effect to realize the applicability of SFM. So, besides the amount of crude protein and amino acid is not enough to broilers. High dirtary fiber content in SFM may affect broilers’ performance. There was no significant effect on feed efficiency when a SFM protein substitute of 20 % SBM protein was used in the growth trial.