| Summary: | 碩士 === 高雄醫學大學 === 藥學研究所 === 88 === 英文摘要
A simple and sensitive high performance liquid chromatographic method is described for the determination of b-hydroxybutyrate in plasma. The b-hydroxybutyrate spiked in plasma, after separation with acetonitrile treatment, was derivatized with strong chromophore reagent, 4-bromomethyl-7- methoxy- coumarin in a homogeneous system, using 15-crown-5 ether as catalyst. The derivative obtained was separated on a RP-C18 column with H2O : methanol (60:40, v/v) as the mobile phase, and 2,2¢-dinitrobiphenyl as the internal standard.
The parameters affecting the derivatization of b-hydroxybutyrate, including catalyst concentration, concentration of alkaline solution, the reaction temperature and reaction time, and the amount of derivatizing agent were investigated. The linear range for the quantitative of b-hydroxybutyrate in human plasma was between 0.05 ~ 2.0 mmol/mL. The intraday relative standard deviation (n = 6) and the interday relative standard deviation (n = 7) were all less than 4.9 % .
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