Detection of Human papillomavirus and Epstein- Bar virus in oral squamous cell carcinoma by using the polymerase chain reaction method

• Main Authors: Chung-Wei Wu, 吳崇維
• Other Authors: TIEN-YU SHIEH
• Format: Others
• Language: zh-TW
• Published: 2000
• Online Access: http://ndltd.ncl.edu.tw/handle/99136747208973921734

碩士 === 高雄醫學大學 === 牙醫學研究所 === 88 === The purpose of this study was to understand the relationship between 50 specimens of oral squamous cell carcinoma or 20 specimens of gingiva and HPV-16 and EBV infection by using polymerase chain reaction method. Our results showed that HPV-16 infection rate of normal gingiva was 10%, and the EBV infection rate is also 10%. The oral cancer patients were divided into one group with betel nut chewing habit and the other group without betel nut chewing habit. HPV-16 DNA could be identified in 15 out of 44 (34%) oral cancer specimens from patients with betel nut chewing habit and in 1 out of 6 (17%) oral cancer specimens from patients without betel nut chewing habit. The overall HPV-16 infection rate in oral cancer specimens was 32% (16/50). There was no significant difference in HPV-16 infection rate between oral cancer specimens and normal gingiva specimens. EBV DNA could be detected in 22 out of 44 (50%) oral cancer specimens from patients with betel nut chewing habit and in 2 out of 6 (33%) oral cancer specimens from patients without betel nut chewing habit. The overall EBV infection rate in oral cancer specimens was 48%. There was a significant difference in EBV infection rate between oral cancer specimens either from patients with betel nut chewing habit or from patients without betel nut chewing habit and normal gingiva specimens. We also found the presence of HPV-16 or EBV DNA in 4 cancerous tissue specimens from metastatic lymph nodes. The HPV-16 DNA positive rate in metastatic lymph nodes was 50% and the EBV DNA positive rate in mrtastatic lymph nodes was 100%. In addition, EBV DNA could be detected in all the recurrent cancers from patients without betel nut chewing habit. Our results suggest that EBV may play a role in oral carcinogenesis in patients with betel nut chewing habit. Furthermore, HPV-16 or EBV may be associated with cervical lymph node metastases and with oral cancer recurrence in patients without betel nut chewing habit. Further studies are needed to prove these hypotheses.