Analysis of metabolites from microbial azo dye decolorization

• Main Authors: Ho Jin-Yen, 何俊穎
• Other Authors: Lin Ping-Jei
• Format: Others
• Language: zh-TW
• Published: 2000
• Online Access: http://ndltd.ncl.edu.tw/handle/98203670416186128972

碩士 === 逢甲大學 === 化學工程學系 === 88 === The biotechnology was one of the most efficient methods in the decolorization of wastewater. The decolorization mechanisms of a reactive azo dye (C.I. Reactive Red 22) from wide-type Pseudomonas luteola strain and recombinant Escherichia coli strain (E. coli NO3) were investigated. The concentration of bacterial cell and Reactive Red 22 was obtained from the ultraviolet-visible photospectrometer. The decolorization products was first separated by reserved phase column of HPLC, then identified by mass spectrometry. It was also found that the time required for complete decolarization of Reactive Red 22 (200ppm) was shorten around 6-7 h with the addition of the metabolites from decolorization of high concentration of Reactive Red 22 (2000ppm) with E. coli NO3.