| Summary: | 碩士 === 中山醫學院 === 生物化學研究所 === 88 === Training increases the antioxidant activity, thereby protecting tissues from the damage caused by free radical. Free radicals are probably involved in the development of post-exercise muscle soreness.
The study in connection with twenty-seven person (13 person football team and 14 person non-sportsman), to differentiate between sportsman group and non-sportsman group, to differentiate between before-exercise group and to run five thousand meter group (exercise group), to different sampling blood. explore sportsman and non-sportsman at before-exercise and ofter-exercise, in blood glutathione (GSH), glutathione peroxidase (GSH-PX), superoxidedismutase (SOD), malondiadehyde (MDA), blood sugar, bloot fat, nitric acid (NO), latic acid (LA) and liver/renal function to change situtation, the purpose of this study is to compare the antioxidant enzyme activities affect in have habit of exercise and height exercise to human.
The change obvious shown in result before/after exercise of the sportsmen group on MDA (1.43±0.55, 0.95±0.54) (P<±0.05), GSH-PX (4.10±1.40, 2.60±0.80) (P<0.02), LAC(4.22±0.79, 12.13±6.1) (P<0.01, creatinine (1.67±0.1, 1.47±0.2) (P<0.01), LDH (371.1±55, 430.5±74) (P<0.05), glucose (85.5±79, 109.0±22) (P<0.05).
The change has shown obviously before/after exercise of non-sportsmen group on SOD (120.95±7.96, 137.38±19.19) (P<0.01), creatinine (1.12±0.1, 1.55±0.2) (P<0.01), uric acid (6.88±1.0, 7.67±1.0) (P<0.05), glucose (78.2±9.1, 125.0±2.6) (P<0.01), Albumin (4.8±0.2, 5.1±0.2) (P<0.01).
T make a comparison between sportsmen group and non-sportsmen group before exercise, it change obviously on GSH (4.16±0.60, 11.70±7.50) (P<0.01), SOD (137.74±14.59, 120.95±7.96) (P<0.01), MDA (1.43±0.55, 0.26±0.02) (P<0.01), NO (6.55±1.351, 23.2±5.12), and also change obvious on (P<0.01), Chol (171±20, 143±28), HDL (63.58±12, 51.28±8.3) (P<0.01), LDL (100±22, 78.5±23) (P<0.02).
And in a comparison between sportsmen group and non-sportsmen group before exercise, it change obviously on GSH (4.53±0.68, 11.79±2.60), MDA (0.95±0.54, 0.25±0.23), GSH-PX (2.60±0.80, 4.00±0.90) (P<0.01), LA (12.13±6.1, 16.3±2.56) (P<0.02), NO (6.146±0.936, 16.3±2.56) (P<0.01), HDL (66±13, 53.6±8.3) (P<0.01), LDL (100±22, 79±22) (P<0.01), Uric acid (8.7±1.7, 7.67±1.0) (P<0.05).
For further examination with CD-1 (ICR) minc, we divided into 4 teams (control, dose-1, dose-2, dose-3) according to the different strength of the sport (swimming) to examine and analyze the items of MDA of serum lipid preoccupation, LA, and organs (e.g. :liver, kidney, heart, and lung), antioxidant enzyme, e.g. :GSH-PX, SOD, and it shown that in the liver, change more obviously (P<0.01) to GSH-PX in control & dose-1, control & dose-2, control & dose-3; and in lung, change more obviously to GSH-DX in dose-1 & dose-2, dose-2 & dose-3; in kidney, change more obviously (P<0.01) to SOD in contrl & dose-2; and change more obviously (P<0.01) for LA in dose-2 & dose-3.
We found the signal transudation protein increased obviously in CD-1 mice’s liver, include P38, JNK1, JNK2, Raf, PKB/Akt, we checked with westernblot test, and we found 4 different sport strength not changed obviously in Raf after exercise, JNK1, JNK2 down gradually, P38 lowered, too, PKB/AKT up gradually. Therefore, signal transudation protein acts an important part in exercises.
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