1.The Morphological Study Of The Biting Midge, Forcipomyia (Lasiohelea) Taiwana (Shiraki) 2.Serological Epidemiology Of The Biting Midge, Forcipomyia (Lasiohelea) Taiwana (Shiraki)

• Main Authors: Shing-Ying Huang, 黃杏英
• Other Authors: Keh-Liang Lin
• Format: Others
• Language: zh-TW
• Published: 2000
• Online Access: http://ndltd.ncl.edu.tw/handle/03405759495746518223

碩士 === 中山醫學院 === 生物化學研究所 === 88 === Part I Forcipomyia (Lesiohelea) taiwana (Shiraki) was discovered and designated in 1913. This species is also called by many other names. The distribution of Shiraki was restricited, however, in recent years it was spread out rapidly and covered almost the whole Taiwan. In this local area (Taichung), this mosquito has been found and become an annoyance to local people. The Environment Protection Authority (EPA) and related departments have conducted the studies about larva breeding, ecology, and control of Forcipomyia (Lesiohelea) taiwana (Shiraki). The result showed that the efficacy of chemical control to larva is not as good as that to the mature mosquito. Since the classification of Forcipomyia spp based on the morphology of mature mosquito is difficult, the study on the morphology of other stages would be of great help in the classification. Therefore, the analysis for morphological characters in all stages are also important to the classification of a bloodsucking midge, Forcipomyia (Lasiohelea) taiwana (Shiraki). The materials in this study are collected from Ta-Ken area in Taichung by human baitting method. The ultra-structure of the midge was studied by means of scanning electron microscopy and analyzed for all stages. The data from this study will be helpful in the classification and chemical control for Forcipomia taiwana. Part II Abstract The major pourpose of the study is to analyze the relationship between the specific IgE of the Forcipomyia taiwana bited voluteers to mosquito allergen and serological epidemiology study. Based on this study, we hope to conduct the studies about Forcipomyia taiwana mosquito allergen locally and provide informations for the futural study. In this study, Western blot and ELISA technique were used to analyze the IgE antibody specific to the whole body extract components of Forcipomyia taiwana from 32 volunteers with common reactions belonging to stage 2 to 5. The result showed that the IgE level is higher in subjects with distinct skin reaction than that with faint skin reaction (P<0.05). Furthermore, it was noticed that the serum IgE level of people lived in Forcipomyia taiwana distributed area is lower than that in non-distributed area. A closed relationship between specific IgE and the skin reaction after exposured to mosquito bite has been observed in this study and so does the frequency of biting. These results revealed the importance of mosquito specific IgE antibodies in pathogenesis of mosquito-bite wheal. This finding is important in potential application for clinical diagnosis.