| Summary: | 碩士 === 中國醫藥學院 === 醫學研究所 === 88 === N-acetylation, a major metabolic pathway for arylamine carcinogen,is catalyzed by cytosolic arylamine N-acetyltransferase(NAT) using acetyl coenzymeA as an acetyl group. Attenuation of NAT activity is associated with several disease processes such as bladder and breast cancer.Butylated Hydroxyanisole (BHA) and Butylated Hydroxytolene(BHT) are synthetic phenolic antioxidants,had been demonstrate to possess cancer preventive properties such as inhibit the liver and bladder cancer development in experimental rats.However,there is no reports to address BHA and BHT affect NAT activity of bladder cancer cells. Thus our proposed study focus on the effect of BHA and BHT on the NAT activity from S-D rat WBC and human bladder cancer cells(T-24).We also examined the Cell Cycle via Flow cytometry,DNA fragmentation,and cell morphology.The result shows The NAT activity of S-D rat WBCand T-24 were supressed by BHA and BHT in a dose-dependent manner.The BHA and BHT also inhibits the S-phase of T-24 cell cycle.We found that BHA and BHT induced T-24 cell DNA fragmentation and Cell morphology destruction.
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