Effect of an urinary preparation on oxidative stress-related parameters in aged or ovariectomized rats.

• Main Authors: I-Ju Chen, 陳臆如
• Other Authors: Wen-Chuan Lin
• Format: Others
• Language: zh-TW
• Published: 2000
• Online Access: http://ndltd.ncl.edu.tw/handle/77228332113479012666

碩士 === 中國醫藥學院 === 中國藥學研究所 === 88 === During aging processes, it is believed that the age-related deterioration of the central nervous system is partly due to the cytotoxicity of reactive oxygen species generated in the brain. Thus, various synthetic or natural compounds having antioxidative properties have been tried to protect against the age-induced deterioration of CNS. In our previous works, we showed that a preparation of human urine (PHU) is a good scavenger of hydroxyl radical. The aim of this experiment was to evaluate the effects of PHU on the age-related parameters, such as lipid peroxidation (LPO), glutathione (GSH). and superoxide dismutase (SOD), in aged male rats and ovariectomized (OVX) rats. In addition, effect of PHU on the osteopenia in OVX rats, and nephrotoxicity induced by cisplatin were also studied. The following results were obtained. 1.The rats (15 months) were received PHU 0.2 or 1.0 g/dl drink water for 8 months. The results showed that MDA concentrations of brain cortex, plasma, aorta, testis were significantly lower in the PHU treated group as compared with the control group. The concentrations of GSH were significantly increased in the liver and kidney of rats treated with PHU. In addition, the diminution of hydroxyproline of skin and kidney in PHU treated rats were also observed. 2.The OVX rats were orally administered PHU for 8 weeks. Fe-independent MDA concentrations in brain cortex, hipocamppus, liver, heart, uterus and adrenal gland, but not in striatum, increased significantly in OVX controls compared to sham-operated rats. Fe-independent MDA concentrations in brain cortex, hipocamppus, striatum and adrenal gland, but not in liver, heart and uterus, decreased significantly in OVX rats treated with PHU compared to those in OVX rats. Fe-dependent MDA concentrations in brain cortex, hippocampus liver, heart and uterus, but not in striatum and adrenal gland, increased significantly in OVX controls compared to sham-operated rats. Fe-dependent MDA concentrations in striatum and hippocampus, but not in brain cortex, liver, heart, uterus and adrenal gland, decreased significantly in OVX rats treated with PHU compared to those in OVX controls. GSH contents and activities of SOD, catalase and GSH-Px of brain cortex were not significantly between the sham-operated group and OVX controls. However, PHU suppressed the activity of GSH-Px, but not SOD and catalase, in the brain cortex of OVX rats. 3.The rats were divided into the sham and OVX groups. The OVX rats were allowed to loss bone for 12 weeks. At 12 weeks post-OVX, the OVX rats were treated with PHU. They were killed 20 weeks postsurgery. OVX rats were osteopenic compared to sham by decreased bone density, ash and calcium content of femur and 4th lumbar spine. PHU decreasede these OVX-induced effects. In addition, PHU increased the weight of vargina and uterus in OVX rats. The data suggest that PHU ameliorated the osteopenia induced by OVX through stimulating the estrogen receptors. 4.Oral administration of PHU for 5 days before and 3 days after single injections of cisplatin (5 mg/kg, i.v. in rats) greatly ameliorated cisplatin-induced nephrotoxicity. PHU-induced amelioration of cisplatin nephrotoxicity was evident by significant reductions in serum BUN and creatinine, and significant improvement in urinary volume and creatinine clearance. PHU increased the GSH concentrations depleted by cisplatin in the renal totoal homogenates and mitochondrial fractions. The findings of this study suggest that increasing effect of PHU on the amounts of GSH be involved in the action of PHU on cisplatin-induced nephrotoxicity. Our results suggest that PHU may delay the aging process by means of its antioxidant effects. This study also demonstrated that PHU is beneficial for osteopenia. In addition, PHU protects against cisplatin-induced renal toxicity, indicating that PHU might have applications for the use of cisplatin in cancer chemotherapy.