The preparation of superficially porous PS-DVB particles and their biochemical applications

• Main Authors: Chen-Yu Kao, 高震宇
• Other Authors: Wen-Chien Lee
• Format: Others
• Language: zh-TW
• Published: 2000
• Online Access: http://ndltd.ncl.edu.tw/handle/47922152614854454934

碩士 === 國立中正大學 === 化學工程研究所 === 88 === Abstract In comparison with totally porous and non-porous cross-linked styrene-divinylbenzene copolymer (PS-DVB) particles, superficially porous PS-DVB particle has the best compromise of the wanted qualities, including the binding capacity, surface functionality and elimination of diffusion resistance. For biological applications, for example, the purification of biomolecules by reversed phase chromatography and enzyme immobilization, the surface hydrophobicity of the particles is one of the most important factors. In this study, the hydrophobic PS-DVB particles having a porous structure with large pores near the periphery of the particles were prepared for biological applications. Styrene-divinylbenzene copolymer particles were prepared via a method of multi-step swelling and polymerization in this research. The influences of the amount of styrene used for preparing polymeric porogen particles and PVP used for copolymerization on the particle morphology and porous structures were studied. The prepared PS-DVB particles were then used as the sorbents for analyzing Penicillin G（Pen G）, 6-APA and PAA by reversed phase chromatography, as well as the supports for immobilization of Candida rugosa lipase. The effects of PVP on these applications were also studied. We also used PS-DVB particles to entrap the nanomagnetic particles via a method of multi-step swelling and polymerization. Increasing the amounts of the styrene and PVP, respectively, used for preparing polymeric porogen and copolymer particles could result in copolymer particles having a higher specific surface area and uniform size distribution, according to the results from the measurement of particle surface area and pore volume by Micromeritics (ASAP 2000) and the SEM observation. We also found that the optimum swelling temperature for monomer absorption prior to polymerization is 30℃. Though the presence of PVP could increase the specific surface area, it also diminished the hyrophobicity of the particles that would reduce the efficiencies for both reversed phase chromatography and immobilization of C. rugosa lipase. Compared with superficially porous PS-DVB particles that contained PVP, the use of particles without PVP as the sorbents of RP-HPLC enhanced the capacity factor of Pen G. Pen G could be effectively separated from the mixture of 6-APA, PAA and Pen G. The immobilization of lipase on the PS-DVB by physical adsorption was achieved. The PS-DVB particles without PVP had a higher specific activity than the ones with PVP. However, the PS-DVB particles with PVP had better mechanic properties, which resulted in a better reproducibility than the ones without PVP. Finally, we found that using a 3-step polymerization without toluene to prepare the magnetic PS-DVB particles could have a better configuration and mono dispersity in a particle size distribution. These magnetic PS-DVB particles are recommended for further biochemical applications.