Analysis of the fengycin synthesis genes fenH-fenP from Bacillus subtilis F29-3

• Main Authors: Chun-Hau Chen, 陳俊豪
• Other Authors: Shin-Tung Liu
• Format: Others
• Language: zh-TW
• Published: 1999
• Online Access: http://ndltd.ncl.edu.tw/handle/33734038568823612197

碩士 === 國立陽明大學 === 微生物暨免疫學研究所 === 87 === Fengycin is an antifungal lipopeptidic antibiotics produced by Bacillus subtilis F29-3. Ealier studies demostrated that fengycin peptide contains 10 amino acids, which is synthesized nonribosomally by peptide synthetases. The purpose of this study is to analyze a 9.6-kb gene cluster in cosmid pFC660, which is potentially involved in the synthesis of the fatty acid in fengycin. DNA sequencing revealed that the region contains nine genes, fenH, fenI, fenJ, fenK, fenL, fenM, fenN, fenO, fenP. Among these genes, fenJ belongs to TetR/AcrR family regulator; fenK, acyl-CoA dehydrogenase; fenL, long-chain fatty acid CoA-ligase; fenM, acetyl-CoA carboxylase; fenN, hydroxymethylglutaryl-CoA lyase; fenO, enoyl-CoA hydratase; fenP, methylmalonyl-CoA decarboxylase. The upstream region of the two genes, fenH, fenJ , were cloned to a promoter-probe vector, pGHL6. Afetr transforming the plasmid into B. subtilis F29-3 and assaying the luciferase activity, only the fenJ upstream region is active. Gene fenJ was overexpressed in E. coli, The overexpression resulted in the formation of insoluble inclusion body. The function of FenJ is now under investigation.