Detection of commonly used sulfonamides in chicken meat from broiler farm

• Main Authors: Re-Shang Chen, 陳瑞祥
• Other Authors: Chaw King Liu
• Format: Others
• Language: zh-TW
• Published: 1999
• Online Access: http://ndltd.ncl.edu.tw/handle/20220826053280187550

碩士 === 國立臺灣大學 === 獸醫學研究所 === 87 === For monitoring four frequently used sulfonamides, sulfadimethoxine (SDM), sulfamonomethoxine (SMM), sulfamethazine (SMT), and sulfaquinoxaline (SQ) in the chicken farm, chicken meat samples were collected from seventeen counties in Taiwan during the period of November, 1997 to March, 1999. After weighted and crushed, the chicken chest meat was extracted and detected for the sulfonamides by using the thin layer chromatography (TLC). Among 347 chicken samples, 5.19﹪(18/347) of them were positive. Moreover, two samples contained both SMM and SMT using TLC method. The TLC-positive samples were further determined their residue quantities by high performance liquid chromatography (HPLC). The percentage of positive samples which contained over the tolerance level 0.1 ppm was 4.03﹪(14/347). HPLC results also showed three samples contained both SMM and SMT while TLC only detected two such samples. The third sample contained 0.006 ppm of SMT, which was lower than TLC detection limit. The detection limits were 0.01 ppm and 0.006 ppm for TLC and HPLC, respectively. The statistical method was employed to analyze the significant difference positive samples with respective to different locations and to different seasons of the year in Taiwan. It was shown that no significant difference was observed between seasons while the expected positive samples from the different locations of Taiwan were significantly different (p<0.01). Furthermore, the difference between the amounts of any two sulfonamides in positive samples was compared with each other. Both SMM vs. SDM and SQ vs. SDM were of significant difference (p<0.05). However, there is no significant difference for either SMM vs. SMT or SQ vs. SMT. In conclusion, the TLC and HPLC methods can be employed in detecting the residue of chicken meat in farm. Results showed that the SMM and SQ are commonly used in chicken farms in Taiwan.