Utilization of ribosomal DNA sequences to identify Chlorella spp. isolates

• Main Authors: Hsiuan-Lin Wu, 吳宣霖
• Other Authors: Ruey-Shyang Hseu
• Format: Others
• Language: zh-TW
• Published: 1999
• Online Access: http://ndltd.ncl.edu.tw/handle/64311262372477654219

碩士 === 國立臺灣大學 === 農業化學研究所 === 87 === Chlorella spp. is one of the microalgae which has been widely used, because Chlorella spp. is a very simple single cell algae. As its morphological characteristics usually change with the environment, it is difficult in identify the species. To determine the relationship between Chlorella spp. strains, this study used PCR techniques to analyze the nuclear-encoded and chloroplast-encoded small-subunit rDNA sequences of 4 strains of Chlorella spp.. These were isolated from different rivers and ponds in Taiwan and Indonesia and then compared with stock strains of Chlorella spp. from the culture collection centers, and published DNA sequence data from Genbank. It was concluded that the isolated strains were mainly attributable to C. sorokiniana, one of the most common species of green algae growing in around 35℃ freshwater ecosystems. Phylogenetic analysis of nuclear-encoded and chloroplast-encoded small-subunit rDNA sequences from round green algae representing the genera Chlorella were found to be of considerable similarity. The analyses also indicated that strain 216 were close to 21 and I. Generally, the chloroplast data sets exhibited in strong support to comparable lineages than the nuclear data sets. 21 and 216 are more close to I. Comparison with morphological and biochemical properties indicate that the result of phylogenetic analyses of rDNA sequences are correct.