| Summary: | 碩士 === 國立臺灣大學 === 農業化學研究所 === 87 === The MRL/lpr mice develop a syndrome similar to human systemic lupus erythematosus (SLE), including production of autoantibodies and formation of immune complexes to cause tissue damage and then renal failure. The purpose of this study was to investigate the effect of vitamin E supplementation on autoimmune disease. MRL/lpr female mice was randomly divided into four groups and when they are fed on an AIN-76 diet containing 5% fresh soybean oil (control)；supplemented with vitamin E at 5 times (E5)、7.5 times (E7.5) and 10 times (E10) of AIN-76 respectively, starting at the age of 3 month. After 2 months experimental diet, eight mice were killed for analysis. The rest of mice were followed up for proteinuria and life span.
The results showed that the growth curve and food efficiency were not significantly different. Supplementation with all-rac-a-tocopheryl acetate elevated the a-tocopherol content in plasma, liver and kidney. However, TBARS values of liver and kidney were not significantly different. Though vitamin E supplementation decreased the PGE2 production of splenocyte, the Th1 cytokine such as IL-2 secretion from splenocyte were significantly lower in E10 group and Th2 cytokine such IL-4、IL-10 were significantly higher. Therefore supplementation with vitamin E neither reduced the autoantibody production nor prolong the life span. The same result was observed in cell cultured that IL-2 secrete has been inhibited by vitamin E supplemented in medium. The IL-2 mRNA expression was also found to be lower when vitamin E was supplemented in the medium. The inhibition effect of vitamin E on IL-2 secretion may be not helpful to Th-2 prone autoimmune disease and remain of interest to be investigated.
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