Chilling Stress Effects the Plastid Development and Gene Expression in mungbean （Vigna radiata L. ）seedlings

• Main Authors: Chen, Shu-Ling, 陳淑玲
• Other Authors: Chen, Yih-Ming
• Format: Others
• Language: zh-TW
• Published: 1999
• Online Access: http://ndltd.ncl.edu.tw/handle/05510974194634430520

博士 === 國立臺灣大學 === 植物學研究所 === 87 === Mungbean ( Vigna radiata L. cv Tainan no. 5 ) is a chilling-sensitive plant. The growth of etiolated mungbean seedlings was very slowly at 10 oC in the light. The leaves continued to grow and finally expranded as those grown at 28oC in the light, but they did not turn to green. The development of plastids into chloroplasts in the etiolated leaves was retarded under chilling in the light condition when leaf ultrastructure was examined under T. E. M.. The synthesis of chlorophyll in the leaves were also retarded by 10 oC-chilling treatment. By SDS-PAGE and 2D-PAGE analyses of total leaf proteins, we found that some PS I and PS II proteins decreased dramatically especially in PSAA, PSAB and other thylakoidal proteins. We also have been screened 10 oC-chilling sensitive genes from vcd01 to vcd10. The length of DNA fragment in vcd01 to vcd10 were 4, 3, 2, 7, 4, 6.5,2.1, 5.6 and 6Kb, respectively. These genes included psaC, psbB, psbC, psbD, psbE, psbF, psbJ, psbN, psbH, psbT, ndhA, ndhB, ndhD, ndhE, ndhG, ndhI, ndhH, petA, petB, petG, rpoA, rpl14, rpl16, rps7, rps15 and ycf5 genes. And all of 10 vcd genes have been sequenced completely. By means of northern blot analysis, the expression of psaA-psaB, ndhB, petB, rpl14, rpl16, and rpoA in the leaves were retarded at 10 oC in the light. Time course studies of retardation gene expression in above genes, from the experimental results showed that the psaA-psaB gene expression reduced immediately after 1 h of 10 oC chilling treatment. The ndhB, petA, rpl14 and rpl16 reduced after 4 h of chilling treatment. But the rpoA gene only reduced slightly after 7 h of chilling treatment. The chilling stress also caused the water deficient in the mungbean seedlings. By means of northern blot analysis, the down-regulated RNA expression in water stress were significantly different from these genes in chilling-sensitive gene expression. The amino acid sequence deduced from the nucleotide sequences of vcd01 to vcd10 shared significant homonology with other plant chloroplast proteins. The base composition, PI value, hydropathy and molecular weight of PSAC, PSBB, PSBE, PSBF, PSBJ, PSBN, PSBT, NDHD, PETA, PETG and YCF5 were also disscused in this study. The availability of vcd01 to vcd10 will facilate the physiological and biological studies in roles of plastid development and the mechanism of these gene expression under 10 oC chilling in the light.