| Summary: | 碩士 === 國立臺灣大學 === 植物學研究所 === 87 === The cell suspension culture of Zinnia elegant was established with the combination NAA 0.5 mg/L and BA 0.01 mg/L in medium. Cells cultured in medium containing 20、50、90、140 mM Cd(NO3)2 for 6 days, the growth of cells decreased to 71.0 %、57.7 %、38.5 % and 30.3 %. The non-protein SH-group was increased in 1 hour with treated 20 mM Cd(NO3)2.
The high pressure liquid chromatography profile of xylem sap showed that the major amino acid was serine which was up to 78% of total amino acids. Plants treated in 10、45 mM Cd(NO3)2 for 5 days, serine in their xylem sap was decreased 41.6％ and 56.5％. Arginine、histidine and valine were decreased in Cd-treated plants. However, leucine、phenylalanine、cysteine、lysine and methionine were increased. Organic acids, such as citric acid and malic acid were not changed with Cd treatment. Potassium ion was highest ( 483.0 mg/mL ) among cation ions in the xylem sap. The content of Zn、Mg、K and Ca were decreased with Cd treatment. However, sodium ion was increased.
In 50 mM CuSO4 treated plants, threonine、arginine、valine、cysteine、lysine and histidine were decreased, but glycine、methionine、leucine and phenylalanine were increased. The content of Zn and K were decreased, but Ca、Na and Mg were increased with copper treatment.
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