Isolation and characterization of two repetitive DNA sequences in Phalaenopsis orchids

• Main Authors: Chien-Hao Huang, 黃建豪
• Other Authors: Chi-Chang Chen
• Format: Others
• Language: zh-TW
• Published: 1999
• Online Access: http://ndltd.ncl.edu.tw/handle/07985466772107743512

碩士 === 國立臺灣大學 === 植物學研究所 === 87 === Two repetitive DNA sequences, PvrⅠand PvrⅡ, were isolated from Phalaenopsis violacea and characterized. The PvrⅠfamily consists of AT-rich regions at 5'' and 3'' ends and 7 bp direct subrepeats (GTAAGCC)13-22. Southern analysis and fluorescence in situ hybridization (FISH) revealed that PvrⅠ is present in P. mannii, P. amboinensis, P. venosa, P. violacea and Doritis pulcherrima and mostly located in the AT-rich subtelomeric heterochromatin of large chromosomes. The PvrⅡ family is present in all eight Phalaenopsis species studied. FISH using one clone (Pvr-95) as a probe showed that PvrⅡis dispersed on all chromosomes of P. venosa.