Recognition of DNA Adducts by Chlorella pyrenoidosa Nucleotide Excision Repair

• Main Authors: Reou-Ching Sheu, 許瑞卿
• Other Authors: Todd Hsu
• Format: Others
• Language: zh-TW
• Published: 1999
• Online Access: http://ndltd.ncl.edu.tw/handle/46078183961348724894

碩士 === 國立海洋大學 === 水產生物技術研究所 === 87 === Nucleotide excision repair (NER), a highly versatile DNA repair mechanism, is capable of removing various types of DNA damage including those induced by UV radiation and chemical mutagens.NER has been well characterized in yeast and mammalian systems but its presence in plants has not been well know in plants. NER in response to UV irradiation, cisplatin damageand mitomycin C (MMC) damage has been detected in soluble extracts of unicellular algae Chlorella pyrenoidosa using a cell-free DNA repair assay in our laboratory. Repair synthesis with irradiated, cisplatin- and MMC-damaged pGEM IV DNAwas dependent on the dose of damage degree.The repair reactions absolutely required the present of cell extract and Mg2+.27mer double-stranded DNA containing adjacent TT in the center was expected to form a thymine dimer after UV irradiation. The repair activity of the extract supernatant after adsorption with UV-irradiated ds27mer is lower than crude extract.