The cytopathogenesis induced by Dengue 2 virus infection in endothelial cells and astrocytes

• Main Authors: CHUNG NAN SUN, 孫忠男
• Other Authors: WANG JAANG JIUN
• Format: Others
• Language: zh-TW
• Published: 1999
• Online Access: http://ndltd.ncl.edu.tw/handle/15651259293089992377

碩士 === 國防醫學院 === 生物及解剖學研究所 === 87 === Dengue virus is a member of the flaviviridae and causes dengue fever, dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). The vascular leakage and hemorrhagic diathesis are the hallmarks of the DHF/DSS, including neurological disorders and encephalitis. It has been reported that endothelial cells may be one of the targets of dengue virus. Dengue virus was also detected in the human brain of a fatal case of DHF, but whether dengue viruses penetrate to brain and cause neuron defects is still unknown. In this study, human umbilical vein endothelial cell line ECV304, human astrocyte cell line HTB-17 and human neuron primary culture were infected with a local isolate strain of DEN-2, PL046, to detect the morphological changes and the viral protein localization. Cytopathological changes, swelled rER and enlarged mitochondria, were observed in cells infected with DEN-2. Virus particles were found in the swelled rER of human neuron primary culture and HTB17.In immunofluorescein and immunogold labeling experiments, ECV304 and HTB-17 show positive results. Viral proteins NS1 and E were localized in the perinuclear regions. DEN-2 infection induced apoptosis was also observed in ECV304 cells by TUNEL methods. According to these results, DEN-2 infection may cause neuron defects via endothelial cells and astrocytes.