| Summary: | 碩士 === 國立成功大學 === 藥理學研究所 === 87 === Our previous report demonstrates that severe gastric mucosal damage can be produced in Lipopolysaccharide (LPS)-intoxicated rat. In the present study, we examined protective effects of several nutrients, including evening primrose oil (EPO), L-arginine, taurine, L-phenylalanine and niacinamide on hemorrhagic ulcers in acid-perfused stomachs of LPS rats.
The male Wistar rats were deprived food for 24 h. Intravenous LPS (3 mg/kg) was challenged 12 h after withdrawal of food. All nutrients were administered before gastric perfusion. Gastric vagotomy was performed, followed by irrigation the stomachs for 3 h with a physiological acid solution containing 100mm HCl and 54 mm NaCl. After 3 hr, rats were killed and gastric ulcer area, vascular permeability (VP), hemoglobin (Hb) content, mucosal glutathione (GSH) level and lipid peroxide (LPO) production were estimated.
The results showed that great gastric mucosal histamine concentration, lipid peroxide productions hemoglobin contents, stomach ulcers and lower glutathione levels were dependent on the doses of LPS challenged. All of these ulcerogenic parameters were significantly attenuated by several nutrients (evening primrose oil 4000 mg/kg, p.o.、L-arginine 500 mg/kg, i.p.、taurine 62.5 mg/rat, i.p.、L-phenylalanine 100 mg/kg, i.p.、niacinamide 500 mg/kg, i.p.).These results indicated that L-phenylalanine、taurine、evening primrose oil or niacinamide ameliorated gastric hemorrhagic ulcers via elevation of mucosal glutathione levels and elimination of mucosal histamine release and oxyradical formation in LPS rats. Intraperitoneal L-arginine also was effective in inhibition. Thus, deficiency of nitric oxide may also be involved in the formation of hemorrhagic ulcers in acid-perfused stomachs of rats.
In conclusion, gastric cytoprotective effects of indicated nutrients may associate with increased mucosal glutathione levels as well as with decreased oxyradical generation and acid back-diffusion in acid-perfused stomachs of endotoxemic rats.
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