| Summary: | 碩士 === 國立中興大學 === 獸醫學系 === 87 === The objective of this study was to determine the concentrations of clenbuterol in equine urine and serum after a continuously oral dose of clenbuterol?HCl.
Horses were fed with 0.8mg of clenbuterol/kg BW twice a day (therapeutic dose). Urine and serum were collected separately after the latest treatment. Blood samples were given at 10min, 15min, 20min, 30min, 1h, 2h, 4h, 6h, 12h, 1d, 2d after dosing; urine were collected at 20min, 30min, 1h, 2h, 4h, 6h, 12h, 1d, 2d after dosing. Samples from treated animal were analysed and the clenbuterol concentrations were comparative to those obtained by ELISA and capillary electrophoresis (CE), using solid phase extraction for sample clean-up.
The highest concentration of clenbuterol found in urine was 72.5 ng/ml (at therapeutic dose) and 2.9 ng/kg in serum (5X therapeutic dose). The initial half-life of serum and urine clenbuterol residues in horses were estimated to be 3.5 and 8 h; the slower elimination for serum and urine approximately 24 and 48 h, respectively. A biphasic pattern of clenbuterol elimination is also characteristic for plasma and urine. Recoveries of clenbuterol from fortified 0.1, 1, 10 ng/ml in serum and urine samples carried through the entire procedure were about 80% relative to pure standards.
ELISA was identified in our reserch to detect clenbuterol in equine serum and urine after oral administration. The SPE method developed in our experiment were appropriate to others in higher recovery rate.
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