RFLP analysis on the genetic relationships of the wild soybeans in Taiwan

• Main Authors: Yueh-Feng Chuang, 莊岳峰
• Other Authors: Fu-Sheng Thseng
• Format: Others
• Language: zh-TW
• Published: 1999
• Online Access: http://ndltd.ncl.edu.tw/handle/29607804734545640193

碩士 === 國立中興大學 === 農藝學系 === 87 === The distribution of two soybean subgenus, Soja and Glycine, intersects on Taiwan. Therefore, a wide variety of wild soybean genetic resources can be found in Taiwan. This research is to understand the relationships between Taiwan wild soybean and its relatives. We used the total DNA of soybean plants as test material. Using two fragments of wild soybean''s mitochontrial DNA, atp6 and coxII, as probes, we performed RFLP analysis and, by the similarity coefficients among the accessions and the results of cluster analysis, investigated their genetic relationships. The result shows that, G. formosana, G. soja, G. gracilis, and G. max in subgenus Soja, the combinations of restriction enzyme－probe including BamHI－atp6, HindIII－coxII, and HindIII－atp6, all produced polymorphic bands. And we used the fragments of G. formosana and G. gracilis amplified by PCR reaction as probe to perform RFLP analysis. The larger than 2 kbp band amplified with the primer OPB-5 of G. gracilis as a probe, produced polymorphic DNA fragments; and the 600 bp band amplified with the primer OPB-12 of G. formosana as a probe, produced a specific band of G. formosana. By cluster analysis, we found that the genetic position of semi-wild G. gracilis lies in between G. max and G. soja, and that G. gracilis can be clustered with G. max. G. formosana lies in a group of its own due to the significant differences in DNA polymorphism and parentage between it and other species in subgenus Soja. In subgenus Glycine, only the DNA digested with BamHI using coxII as a probe to yield polymorphic hybridization production, which is also exclusive to G. dolichocarpa. The other combinations, including the 200 bp band amplified with the primer OPB-7 of G. dolichocarpa as a probe, produced no hybridization production. The results shows that there exists differences in DNA sequence among G. dolichocarpa, G. tomentella, and G. tabacina.