Physiological Study on Hydrogen Metabolism of Cyanobacterium Anabaena CH1 and CH2

• Main Authors: Wei-Fen Sheu, 許偉芬
• Other Authors: Pei-Chung Chen
• Format: Others
• Language: zh-TW
• Published: 1999
• Online Access: http://ndltd.ncl.edu.tw/handle/28270756808535900494

碩士 === 國立中興大學 === 植物學系 === 87 === Hydrogenase activity in N2-fixing cyanobacterium Anabaena CH1 and CH2 have different physiological reactions in different environments, The growth rates of Anabaena CH1 and CH2 reduces with the decreasing light intensities in different illumination degree. In the dark, both Anabaena CH1 and CH2 do not grow. The hydrogen formation activity of the extract of Anabaena CH1 were lower in 25℃ than 4℃. The hydrogen formation activity of the extract of Anabaena CH2 did not exhibit hydrogenase activity in 25℃ with different illumination degree. The reversible hydrogenase activity of Anabaena CH1 and CH2 were increasing with the decreasing light intensities. The reversible hydrogenase activities of both Anabaena CH1 and CH2 were very low in air and in 240 mole m-2s-1 light intensity. Reprocess in dark 24hr, reversible hydrogenase activity were increasing several times. In light intensity were 240 mole m-2s-1, Anabaena CH2 did not exhibit uptake hydrogenase activity. Glycogen reduces with the decreasing light . Uptake hydrogenase activity could be detected with decreasing levels of glycogen. Uptake hydrogenase activity also be detected in air and in light intensity were 240 mole m-2s-1. Anabaena CH2 by prolonged incubation under dark, the uptake hydrogenase activity was increasing. The results showed that the carbohydrate level present in N2-fixing cyanobacterium is negatively correlated with hydrogenase activity. It''s means that there would has low levels of the lower carbohydrate storage materials when hydrogenase was induced.