| Summary: | 碩士 === 國立中興大學 === 化學工程學系 === 87 === In this study, we used a medical grade polyurethane, Pellethane 2363-80A, to fabricate sponge type small-diameter vascular grafts by a salt casting technique. The grafts were compliance-matched (with a storage modulus was 0.53±0.08 MPa) by a salt casting technique. The inner surface of grafts was coated with a thin layer (~ 40 μm) of gelatin crosslinked by epoxide. Then a special RGD-containing peptide was coated on the gelatin layer.
The platelet adhesion and activation was evaluated by using platelet rich plasma (PRP). It was observed that the normalized number of activated platelets on the best modified surface (i.e. that modified by both gelatin and RGD) was only about a half of that on the surface without modification. Therefore, surface modification by gelatin and RGD indeed increased the blood compatibility.
After endothelial cells were seeded onto the inner surface of the vascular grafts, the grafts were perfused in vitro and the cell retention was measured. The cell number on the surface modified by gelatin and RGD after in vitro perfusion was enhanced by a factor of three.
The primary endothelial cells were isolated from canine external jugular vein, cultured and seeded onto the vascular graft. The cell coverage was observer by staining. The primary cells also had a high cell coverage on the surface modified by gelatin and RGD.
We believed that these experimental results suggested patency of such grafts when implanted in the animal model in the future.
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