Effects and Mechanisms of glyceryl nonivamide and sodiumnonivamide acetate on the Tracheal Smooth Muscle in Guinea Pig

碩士 === 高雄醫學院 === 醫學研究所 === 87 === The effects of glyceryl nonivamide (GLNVA) and sodium nonivamide acetate (SNA) on isolated tracheal smooth muscle of guinea-pig in vitro and the desensitizing effect of GLNVA and SNA in anesthetized guinea-pig airways in vivo were first investigated. Both...

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Main Authors: Chin-hsiu Lin, 林錦秀
Other Authors: Ing-Jun Chen
Format: Others
Language:zh-TW
Published: 1999
Online Access:http://ndltd.ncl.edu.tw/handle/q3hub8
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spelling ndltd-TW-087KMC005340212018-04-10T17:13:41Z http://ndltd.ncl.edu.tw/handle/q3hub8 Effects and Mechanisms of glyceryl nonivamide and sodiumnonivamide acetate on the Tracheal Smooth Muscle in Guinea Pig Glyceryl nonivamide及sodium nonivamide acetate對於天竺鼠氣管平滑肌之作用及機轉 Chin-hsiu Lin 林錦秀 碩士 高雄醫學院 醫學研究所 87 The effects of glyceryl nonivamide (GLNVA) and sodium nonivamide acetate (SNA) on isolated tracheal smooth muscle of guinea-pig in vitro and the desensitizing effect of GLNVA and SNA in anesthetized guinea-pig airways in vivo were first investigated. Both GLNVA (0.01-10 uM) and SNA (0.01-100 uM) produced concentration-dependent contraction in the isolated guinea-pig trachea, and this effect were inhibited by pretreatment with capsazepine (10 uM), ruthenium red (10 uM), theophylline (10 uM), or sensory denervation, respectively. On the other hand, at higher concentration, GLNVA 5 uM could produce relaxation on carbachol-induced contracted tracheal smooth muscle after sensory denervation. Moreover, this relaxation effect was completely blocked by indomethacin (10 uM) and partially blocked by TEA (tetraethylammonium, non-selective potassium channel blocker, 10-2 M), glibenclamide (10 uM), theophylline (10 uM), 8-PT (8-phenyltheophylline, adenosine A1 receptor antagonist, 10 uM) and DMPX (3,7-dimethyl-1-propargylxanthine, adenosine A2 receptor antagonist, 10 uM) . After long-term pretreatment of GLNVA and SNA (105.6 mg, 7 days, s.c.), GLNVA significantly inhibited ovalbumin- or capsaicin-induced tracheal smooth muscle contraction, but SNA did not. In anesthetized guinea-pig, long term treatment of GLNVA and SNA, also significantly inhibited capsaicin-induced pulmonary dysfunction. The distribution of SP-like immunoreactive nerves in guinea pig airway was also displayed in this study. In the present study we find that capsaicin and the nonpungent capsaicin analogue GLNVA can deplete the SP from sensory nerve in guinea pig trachea. In conclusion, the present data indicated that, capsaicin, GLNVA and SNA caused chemical desensitization of sensory nerve in guinea-pig tracheal smooth muscle and renders them insensitive to further exposure of capsaicin. The tracheal hyperresponsivess of guinea-pig induced by ovalbumin were attenuated following chronic treatment with capsaicin, GLNVA and SNA which destroys sensory C-fiber. GLNVA induced- relaxation effect was mediated via the activation of ATP-sensitive K+ channel opening, adenosine receptor modulation and release of endogenous prostanoids of the smooth muscle. Ing-Jun Chen Yi-Ching Lo 陳英俊 羅怡卿 1999 學位論文 ; thesis 73 zh-TW
collection NDLTD
language zh-TW
format Others
sources NDLTD
description 碩士 === 高雄醫學院 === 醫學研究所 === 87 === The effects of glyceryl nonivamide (GLNVA) and sodium nonivamide acetate (SNA) on isolated tracheal smooth muscle of guinea-pig in vitro and the desensitizing effect of GLNVA and SNA in anesthetized guinea-pig airways in vivo were first investigated. Both GLNVA (0.01-10 uM) and SNA (0.01-100 uM) produced concentration-dependent contraction in the isolated guinea-pig trachea, and this effect were inhibited by pretreatment with capsazepine (10 uM), ruthenium red (10 uM), theophylline (10 uM), or sensory denervation, respectively. On the other hand, at higher concentration, GLNVA 5 uM could produce relaxation on carbachol-induced contracted tracheal smooth muscle after sensory denervation. Moreover, this relaxation effect was completely blocked by indomethacin (10 uM) and partially blocked by TEA (tetraethylammonium, non-selective potassium channel blocker, 10-2 M), glibenclamide (10 uM), theophylline (10 uM), 8-PT (8-phenyltheophylline, adenosine A1 receptor antagonist, 10 uM) and DMPX (3,7-dimethyl-1-propargylxanthine, adenosine A2 receptor antagonist, 10 uM) . After long-term pretreatment of GLNVA and SNA (105.6 mg, 7 days, s.c.), GLNVA significantly inhibited ovalbumin- or capsaicin-induced tracheal smooth muscle contraction, but SNA did not. In anesthetized guinea-pig, long term treatment of GLNVA and SNA, also significantly inhibited capsaicin-induced pulmonary dysfunction. The distribution of SP-like immunoreactive nerves in guinea pig airway was also displayed in this study. In the present study we find that capsaicin and the nonpungent capsaicin analogue GLNVA can deplete the SP from sensory nerve in guinea pig trachea. In conclusion, the present data indicated that, capsaicin, GLNVA and SNA caused chemical desensitization of sensory nerve in guinea-pig tracheal smooth muscle and renders them insensitive to further exposure of capsaicin. The tracheal hyperresponsivess of guinea-pig induced by ovalbumin were attenuated following chronic treatment with capsaicin, GLNVA and SNA which destroys sensory C-fiber. GLNVA induced- relaxation effect was mediated via the activation of ATP-sensitive K+ channel opening, adenosine receptor modulation and release of endogenous prostanoids of the smooth muscle.
author2 Ing-Jun Chen
author_facet Ing-Jun Chen
Chin-hsiu Lin
林錦秀
author Chin-hsiu Lin
林錦秀
spellingShingle Chin-hsiu Lin
林錦秀
Effects and Mechanisms of glyceryl nonivamide and sodiumnonivamide acetate on the Tracheal Smooth Muscle in Guinea Pig
author_sort Chin-hsiu Lin
title Effects and Mechanisms of glyceryl nonivamide and sodiumnonivamide acetate on the Tracheal Smooth Muscle in Guinea Pig
title_short Effects and Mechanisms of glyceryl nonivamide and sodiumnonivamide acetate on the Tracheal Smooth Muscle in Guinea Pig
title_full Effects and Mechanisms of glyceryl nonivamide and sodiumnonivamide acetate on the Tracheal Smooth Muscle in Guinea Pig
title_fullStr Effects and Mechanisms of glyceryl nonivamide and sodiumnonivamide acetate on the Tracheal Smooth Muscle in Guinea Pig
title_full_unstemmed Effects and Mechanisms of glyceryl nonivamide and sodiumnonivamide acetate on the Tracheal Smooth Muscle in Guinea Pig
title_sort effects and mechanisms of glyceryl nonivamide and sodiumnonivamide acetate on the tracheal smooth muscle in guinea pig
publishDate 1999
url http://ndltd.ncl.edu.tw/handle/q3hub8
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