Diversity of classic anti-neutrophil cytoplasmic antibody (cANCA) and perinuclear anti-neutrophil cytoplasmic antibody (pANCA) in vasculitides and autoimmune diseases

• Main Authors: Fu Huey Ling, 傅惠玲
• Other Authors: 蔡嘉哲
• Format: Others
• Language: zh-TW
• Published: 1999
• Online Access: http://ndltd.ncl.edu.tw/handle/59703575428146229480

碩士 === 中山醫學院 === 生物化學研究所 === 87 === Objective. To characterize antigens recognized by classic anti-neutrophil cytoplasmic antibody (cANCA) and perinuclear anti- neutrophil cytoplasmic antibody (pANCA) in vasculitis and autoimmune disease. Methods. A total of 160 serum samples from patients with different clinical manifestations in whom systemic vasculitides were suspected and 40 control samples were tested for cANCA and pANCA by indirect immunofluorescence (IIF). All available sera were further tested by enzyme-linked immunosorbent assay (ELISA) for the presence of antibodies to proteinase 3 (PR3), myeloperoxidase (MPO), lactoferrin (LF) and lysozyme (LZ). In addition, sera from 110 patients with systemic lupus erythematosus (SLE) and 51 patients with rheumatoid arthritis (RA) were also tested by ELISA for antibodies to PR3, MPO, LF and LZ. Results. ANCA were detected in 81 of the 160 sera by IIF. A cANCA pattern of fluorescence was detected in 21 sera and a pANCA pattern was observed in 60 sera. By ELISA testing, of the 21 sera with cANCA, 12 sera reacted to PR3, 4 to LF, 4 to LZ and 3 to MPO. Of the 60 sera with pANCA, 15 sera reacted with MPO, 13 to PR3, 8 to LF and 4 to LZ. The prevalence of ANCA antigenic specificities in SLE were anti-PR3= 0%; anti-MPO= 1%; anti-LF= 27.2%; anti-LZ= 29.1%. The prevalence of ANCA antigenic specificities in RA were anti-PR3= 5.9%; anti-MPO= 2%; anti-LF= 7.8%; anti-LZ= 3.9%. Conclusion. cANCA and pANCA are reacted with diverse cytoplasmic antigens of the neutrophils. Antibodies to PR3 and MPO are not common in sera from patients with SLE and RA. Sera from patients with SLE contain more anti-LF and anti-LZ antibodies than anti-PR3 and anti-MPO antibodies. Rabbits anti-MPO and anti-LF antiserum both showed pANCA staining pattern and anti-HMG 1? showed a cANCA staining pattern in indirect immunofluorescence.