Semiquantitative Analysis of mRNA Level for Matrinx Molecules in Torn Rorator Cuff Tendon Using Reverse Transcription - Polymerase Chain Reaction

• Main Authors: Chun-Yi Lee, 李俊億
• Other Authors: Horng-Chaung Hsu
• Format: Others
• Language: zh-TW
• Published: 1999
• Online Access: http://ndltd.ncl.edu.tw/handle/61904955236916284458

碩士 === 中國醫藥學院 === 醫學研究所 === 87 === Biopsy specimens of torn rotator cuff tendons from 13 patients with complete-thickness tears and 3 patients with incomplete-thickness tears were obtained during surgery. Three macroscopically normal rotator cuff tendons were obtained to serve as normal controls. RNA was isolated from each tendon, and expression of type-I collagen, type-III collagen, biglycan and decorin was evaluated by quantitative reverse transcription-polymerase chain reaction with use of b-actin as the housekeeping gene. Data for complete-thickness and incomplete-thickness tears were compared statistically with normal controls. The results revealed that both normal and torn tendons contained cells that express mRNA for all molecules studied. However, cells in complete-thickness and incomplete-thickness tears express much lower for type-I collagen (P<0.001), type-III collagen (P<0.01), biglycan (P<0.05) and ratio of Biglycan and decorin (P<0.05)than do in normal rotator cuff tendon, but no significant difference for decorin was noted. There is no significant difference between duration of pain, torn size, age and gender in complete-thickness tears. These results demonstrate that complete-thickness tears may not possess an intrinsic healing capacity whenever the duration of pain has persisted and whatever tendons are incomplete-thickness or complete-thickness tears. The results also demonstrate that torn tendons may resist less compression force and shearing force. The torn tendons may be at disuse stage and healing process can not be expected, even incomplete-thickness tears. To achieve complete healing, torn tendons should be aided by surgical repair.