Ⅰ: Simultaneous Assay of Cationic Disinfectant in Cosmetics and Pharmaceutical Products Using HPLC Equipped with a Silica Gel Column and an Aqueous Mobile Phase. Ⅱ: HPLC Separation and Identificatio

• Main Authors: Wu Kun Shan, 吳坤憲
• Other Authors: Chau-Yang Chen
• Format: Others
• Language: zh-TW
• Published: 1998
• Online Access: http://ndltd.ncl.edu.tw/handle/58028693554568998303

碩士 === 台北醫學院 === 藥學研究所 === 86 === Ⅰ. Simultaneous Assay of Cationic Disinfectant in Cosmetics and Pharmaceutica l Products Using HPLC Equipped with a Silica Gel Column and an Aqueous Mobile PhaseThis report reveals that a simple, rapid and reliable high performance li quid chromatographic (HPLC) method has been developed. This method provides si multaneously to identify and quantify two cationic disinfectants: benzalkonium chloride and chlorhexidine gluconate in cosmetics; as well as four other cati onic disinfectants: domiphen bromide, benzethonium chloride, cetylpyridinium c hloride and dequalinium chloride in pharmaceuticals. The HPLC analytical metho d involves the use of a silica gel column and an aqueous mobile phase. The dis infectants are separated by isocratic chromatography on a m-porasil silica gel column (3.9 * 300 nm) using an UV detector at 262 nm. Six standard solutions of the cationic disinfectants with different concentrations were found to have excellent linear relationships (r > 0.999). The results obtained are not inf luenced by the vehicle incorporated in cosmetics or pharmaceuticals by using this analytical meⅡ. HPLC Separation and Identification of Illegal Coal Tar D yes in Mucous Cosmetics Illegal coal tar dyes blended in mucous cosmetics cou ld induce toxic and carcinogenic threats. In accordance with Chinese National Standard, the methods of hygienic test adopted for analyzing organic coloring materials in cosmetics are paper chromatography (PC) and thin layer chromatogr aphy(TLC). This study was using HPLC method to promote the analysis efficienc y in separating and identifying the illegal coal tar dyes in mucous cosmetics. The HPLC column was Cosmosil 5C18-AR-Ⅱ,mobile phase comparitions were as fo llows:(a) for hydrophilic coal tar dyes :0.07M ammonium aceate(including 0.01M tetrabutylammonium bromide ) : acetonitrile : methanol=55:35:10. This method is capable of separating 16 coal tar dyes. (b) for hydrophobic coal tar dyes, methanol:water=95:5, a total of 6 coal tar dyes could be separated. Visible wa velength photodiode array detector was used for detection. The minimum detecta ble concentration was 0.05μg/mL. The results clearly shows that a total of 2 2 illegal coal tar dyes can be separated and identified successfully as report ed above.