The effects of carotenoids on the antioxidative status and lipid peroxidation in primary rats hepatocytes

• Main Authors: Hsiang Yi-Jung, 項益容
• Other Authors: Cheng Hsing-Hsien
• Format: Others
• Language: zh-TW
• Published: 1998
• Online Access: http://ndltd.ncl.edu.tw/handle/44241743656984381480

碩士 === 台北醫學院 === 保健營養學系 === 86 === The studies have shown the important roles of carotenoids in improving antioxidative capacity and decreasing lipid peroxidation, indicating carotenoids may decrease the incidence of cardiovascular disease and cancer related to free radicals and reactive oxygen species. However, it was reported that no effect of beta-carotene on enhancing antioxidative status and lowering lipid peroxidation in vitro was observed. The effects of carotenoids on antioxidative status and lipid peroxidation in rat hepatic explants were less studied. Therefore, it was hypothesized that carotenoids could protect rat hepatic explants from peroxidation in vitro. Furthermore, this study investigated the effects of carotenoids on antioxidant enzyme activities and lipid peroxidation after the addition of hydrogen peroxide as an oxidative inducer to increase oxidative stress in the cells. Firstly, the results showed that lactate dehydrogenase (LDH) leakage was increased with increased culture time, suggesting cell viability of the hepatocytes was decreased under the normal condition. The time course study found that LDH leakage was significantly increased after 24-h beta- carotene treatment. Secondly, the dose and timing were determined by the results of lipid peroxidation and antioxidative status after the addition of 0~100 uM hydrogen peroxide. The dose and time course studies indicated that the treatment of 10 uM hydrogen peroxide for 10 min could induce the antioxidative enzyme activities. Thirdly, the effects of beta- carotene, canthaxanthin, alpha-carotene, lutein, retinol or alpha-tocopherol on lipid peroxidation and antioxidative status after 20-h incubation with or without pretreatment of 10 uM hydrogen peroxide for 10 min were investigated. The data indicated that various antioxidative status was observed after the addition of different carotenoids. Canthaxathin, lycopene, or lutein increased the activities of superoxide dismutase, glutathione reductase, and glutathione S-transferase as compared to bata- carotene. In addition, carotenoids had a tendency to inhibit lipid peroxidation. It was also found that beadlet carotenoids, especially anthaxanthin and lutein, had better antioxidative effectiveness compared to oil-soluble carotenoids (crystalline form).