| Summary: | 碩士 === 淡江大學 === 化學學系 === 86 === A glucose biosensor with exceptional linearity was fabricated by
mixing withglucose oxidase and carbon ink. The ruthenium oxides
were prepared with glucoseoxidase was modefied with Nafion
membrane onto the ink surface. Subsequently, the glucose can be
measured at +0.2 V(vs. Ag/AgCl), in 0.05 M pH 7.4
phosphatebuffer solution, 400 rpm, 25 C. The linearly of the
response at best is up to 50 mM (with correlation coefficient
0.99992). The sensitivity of the electrodeis 366 nA/mM.cm2. The
estimated detection limit (S/N=3) is 4.36x10-4 M. The response
time t10%-90% is 66 seconds. The standard deviation of twenties
repetivedetection of 5 mM glucose is 1.894%. There is no
significant interference observedfrom the addition of 0.1 mM
ascorbic acid, 0.4 mM uric acid, 0.2 mM cystine, glutamicacid,
histidine, tyrosine, arabinose, fucose, galatose, lactose,
mannose, raffinose,stachyose, xylose and 10 mM alcohol at
presence or unpresence of 5.5 mM glucose.
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