The research of using the distiller'' grain for the fruit body culture of Ganoderma lucidum in solid state fermentation and thin stillage for the mycelial culture in submerged culture

• Main Authors: Chen,Hang-Ming, 陳漢銘
• Other Authors: Yang,Fan-Chiang
• Format: Others
• Language: zh-TW
• Published: 1998
• Online Access: http://ndltd.ncl.edu.tw/handle/44403414590720410467

碩士 === 東海大學 === 化學工程學系研究所 === 86 === The main objective of this research is to investigate the feasibility of using the stillage grain for the fruit body culture of Ganoderma lucidum in solid state fermentation and thin stillage for the mycelial culture in submerged culture. The solid state fermentations were carried out in test tubes or propylene bags. The additions of NH4H2PO4 and CaCO3 were demon-strated to be in favor of the mycelial growth. The mixtures of wood chip (80%) and stillage grain (20%) at the moisture level of 60% were deter-mined to be optimal for the fruiting body culture in propylene bags. The compositions of fruiting body were also analyzed and compared with the control. Liquid cultures were performed in shaken flasks or fermenters. Different operating modes, including repeated batch and continuity, have been tested. In a repeated-batch culture using a 5-liter fermenter the concentration of polysaccharide reached 18.26mg/dL and the yields were estimated to be around 30-50mg/g glucose. In a continuous operation using a 12-liter fermenter the polysaccharide concentration and the yield were 13.5 mg/dL and 22 mg/g glucose at dilution rate (0.005hr-1). Con-tinuous production of polysaccharide was proved to be feasible.-1 -aThe research of using the distiller'' grain for the fruit body culture of Ganoderma lucidum in solid state fermentation and thin stillage for the mycelial culture in submerged culture